Fig 1: Lipoxins reduce obesity-related inflammation in human adipose tissue explants from patients with elevated CRP levels(A) Schematic illustration of the experimental approach, where adipose tissue explants from patients were treated with vehicle, LXA4, or LXB4 for 6 h, after which the tissue mRNA and supernatant cytokine levels were analyzed and immunohistochemistry was performed.(B–F) Macrophage markers were quantified by mRNA expression (B–D: M2 markers MRC1, CD163, and M1 marker ITGAX) and immunofluorescence co-localization (E–F: CD68 pan-marker, CD11c M1 marker, and CD206 M2 marker). (E) CD68: green in overlay, CD11c: red in overlay, CD206: blue in overlay, DAPI: white in overlay. Arrow highlights CD206+,CD68+ (M2) macrophages, arrowhead highlights CD11c+,CD68+ (M1) macrophages. Scalebar = 20µm.(G–K) Cytokine and adipokine expression. G: Interleukin (IL)-10 mRNA, H-K: Supernatant levels of TNF-a, IL-6, adiponectin and leptin.(L) mRNA expression of the LXA4 receptor FPR2. Dashed line represents the patients’ individual baseline expression set to 100%, in non-responder and responder groups, respectively. Statistical analysis was done by Kruskal-Wallis test with Dunn’s post-hoc comparison. Data are presented as mean ± SEM. *p < 0.05, **p < 0.01, ***p < 0.001. Figure relates to Figures S1 and S3.
Fig 2: Prognostic impact of CD4+ T cells and the presence of CD103+CD11c+ cells in patients with CD103high and CD8low. A, Kaplan‐Meier plots using the log‐rank test for OS according to the number of CD4+ T cells in patients with CD103high and CD8low. Patients with CD4low tended to have a better prognosis than those with CD4high (P = .060). B, Representative images of immunofluorescence staining of CD103 (red), CD11c (green), and DAPI (blue) are shown. CD103+CD11c+ cells (white arrow) were rarely observed
Fig 3: (A) Representative images of immunohistochemical analyses using anti‐CD4 and anti‐CD103 antibodies. Scale bar, 100 μm. (B) Representative images of immunofluorescence staining of CD11c (green), CD103 (red), and DAPI (blue). Scale bar, 100 μm. (C) Representative images of immunohistochemical analyses using anti‐HLA class I/II and anti‐CD103 antibodies. Scale bar, 100 μm.
Supplier Page from Abcam for Anti-CD11c antibody [ITGAX/1243]